Differential CNS expression of alternative mRNA isoforms of the mammalian genes encoding cAMP-specific phosphodiesterases

Bolger, G. B., Rodgers, L., Riggs, M. (November 1994) Differential CNS expression of alternative mRNA isoforms of the mammalian genes encoding cAMP-specific phosphodiesterases. Gene, 149 (2). pp. 237-44. ISSN 0378-1119 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7958996
DOI: 10.1016/0378-1119(94)90155-4

Abstract

To study alternative splicing and tissue-specific expression of the mammalian genes encoding type-IV cAMP-specific phosphodiesterases, which are homologs of the dnc learning and memory gene of Drosophila melanogaster, we cloned seven cDNAs from four rat loci (PDE1, PDE2, PDE3 and PDE4) homologous to dnc. The deduced amino-acid sequences of the proteins encoded by the rat loci were shown to have a 1:1 correspondence with those encoded by the four human dnc homologs. The proteins encoded by at least one cDNA from each of the four rat loci contained novel N-terminal upstream conserved regions (UCR1 and UCR2), described previously in proteins encoded by the human dnc homologs and by dnc. cDNAs from three of the rat loci (PDE2, PDE3 and PDE4) had a structure consistent with alternative splicing of the 5' coding regions of their respective mRNAs. UCR1, and in one case a portion of UCR2, were absent in one of the alternatively spliced transcripts from these three loci. RNase protection analysis showed that the rat PDE3 and PDE4 loci were each expressed at relatively constant levels in multiple regions of the brain, while PDE2 transcripts were more abundant in temporal cortex and brainstem. One of the alternatively spliced mRNAs from the PDE4 locus was relatively more abundant in temporal cortex and cerebellum. One alternatively spliced transcript from the PDE3 locus was expressed more abundantly in parietal cortex. Both of the alternatively spliced transcripts from the human DPDE4 locus (the homolog of rat PDE4) were expressed in temporal cortex.

Item Type: Paper
Uncontrolled Keywords: 3',5'-Cyclic-Nucleotide Phosphodiesterase/ biosynthesis/genetics Alternative Splicing Amino Acid Sequence Animals Brain/ enzymology Brain Stem/enzymology Cerebellum/enzymology Comparative Study Conserved Sequence DNA, Complementary/biosynthesis/chemistry Drosophila melanogaster/genetics Gene Expression Genes, Insect Humans Isoenzymes/ biosynthesis/genetics Learning Memory Molecular Sequence Data Organ Specificity Phylogeny RNA, Messenger/biosynthesis Rats Research Support, U.S. Gov't, P.H.S. Sequence Homology, Amino Acid Temporal Lobe/enzymology Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor > Cyclic AMP
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: Matt Covey
Date: 18 November 1994
Date Deposited: 25 Aug 2015 18:34
Last Modified: 25 Aug 2015 18:34
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31427

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