pMPY-ZAP: a reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae

Schneider, B. L., Steiner, B., Seufert, W., Futcher, A. B. (1996) pMPY-ZAP: a reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae. Yeast, 12 (2). pp. 129-34. ISSN 0749-503X (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8686376
DOI: 10.1002/(SICI)1097-0061(199602)12:2<129::AID-YEA891>3.0.CO;2-O

Abstract

Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Genetic Markers Molecular Sequence Data Mutagenesis, Insertional/ methods Orotidine-5'-Phosphate Decarboxylase/genetics Polymerase Chain Reaction Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ genetics Selection (Genetics) Transformation, Genetic
Subjects: organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
CSHL Authors:
Communities: CSHL labs > Futcher lab
Depositing User: Kathleen Darby
Date Deposited: 13 May 2014 15:54
Last Modified: 13 May 2014 15:54
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30105

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