Determination of Arabidopsis floral meristem identity by AGAMOUS

Mizukami, Y., Ma, H. (1997) Determination of Arabidopsis floral meristem identity by AGAMOUS. Plant Cell, 9 (3). pp. 393-408. ISSN 1040-4651 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9090883
DOI: http://www.plantcell.org/content/9/3/393.abstract

Abstract

Determinate growth of floral meristems in Arabidopsis requires the function of the floral regulatory gene AGAMOUS (AG). Expression of AG mRNA in the central region of floral meristems relies on the partially overlapping functions of the LEAFY (LFY) and APETALA1 (AP1) genes, which promote initial floral meristem identity. Here, we provide evidence that AG function is required for the final definition of floral meristem identity and that constitutive AG function can promote, independent of LFY and AP1 functions, the determinate floral state in the center of reproductive meristems. Loss-of-function analysis showed that the indeterminate central region of the ag mutant floral meristem undergoes conversion to an inflorescence meristem when long-day-dependent flowering stimulus is removed. Furthermore, gain-of-function analysis demonstrated that ectopic AG function results in precocious flowering and the formation of terminal flowers at apices of both the primary inflorescence and axillary branches of transgenic Arabidopsis plants in which AG expression is under the control of the 35S promoter from cauliflower mosaic virus. Similar phenotypes were also observed in lfy ap1 double mutants carrying a 35S-AG transgene. Together, these results indicate that AG is a principal developmental switch that controls the transition of meristem activity from indeterminate to determinate.

Item Type: Paper
Uncontrolled Keywords: AGAMOUS Protein, Arabidopsis Arabidopsis/genetics/ growth & development/metabolism DNA-Binding Proteins/ biosynthesis/genetics Gene Expression Regulation, Plant Genes, Plant Genes, Regulator Meristem/ultrastructure Microscopy, Electron, Scanning Mutation Phenotype Plant Proteins/ biosynthesis/genetics RNA, Messenger/biosynthesis Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Transcription, Genetic
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
Investigative techniques and equipment > microscopy
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs
Depositing User: Kathleen Darby
Date Deposited: 08 May 2014 17:01
Last Modified: 08 May 2014 17:01
PMCID: PMC156926
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29978

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