Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity

Tansey, William P., Herr, Winship (February 1997) Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity. Science, 275 (5301). pp. 829-31. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9012349
DOI: 10.1126/science.275.5301.829

Abstract

Interaction between the TATA box-binding protein TBP and TFIIB is critical for transcription in vitro. An altered-specificity TBP-TFIIB interaction was rationally designed and linked in sequence to an altered-specificity TATA box-TBP interaction to study how TBP and TFIIB function together to support transcription in human cells. The activity of this altered-specificity TATA-TBP-TFIIB array demonstrated that many activators use the known TBP-TFIIB interaction to stimulate transcription. One activator, however, derived from a glutamine-rich activation domain of Sp1, activated transcription independently of this interaction. These results reveal that selectivity in activator function in vivo can be achieved through differential use of TBP and TFIIB.

Item Type: Paper
Uncontrolled Keywords: DNA-Binding Proteins/chemistry/genetics/ metabolism Fungal Proteins/metabolism Genes, Reporter Genes, fos Hela Cells Humans Mutagenesis, Site-Directed Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sp1 Transcription Factor/metabolism TATA Box TATA-Box Binding Protein Trans-Activators/metabolism Transcription Factor TFIIB Transcription Factors/chemistry/genetics/ metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
CSHL labs > Tansey lab
Depositing User: Kathleen Darby
Date: 7 February 1997
Date Deposited: 08 May 2014 18:27
Last Modified: 08 May 2014 18:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29969

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