Methods for producing probes capable of distinguishing DNA from related sources. United States Patent 6159713

Michael Wigler (2000) Methods for producing probes capable of distinguishing DNA from related sources. United States Patent 6159713. .

URL: http://www.freepatentsonline.com/6159713.html

Abstract

Methodology is provided for developing probes for identifying sequence differences between two related DNA populations, sets of DNA fragments or collections of restriction-endonuclease-cleaved DNA or cDNA. The method employs an initial stage to obtain a representation of both DNA populations, namely using the PCR to produce relatively short fragments, referred to as amplicons. Tester amplicons containing target DNA, sequences of interest, are ligated to adaptors and mixed with excess driver amplicons under melting and annealing conditions, followed by PCR amplification. The process may be repeated so as to greatly enrich the target DNA. Optionally, the target DNA may then be cloned and the DNA used as probes.

Item Type: Patent
Subjects: patents
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: Matt Covey
Date: 2000
Date Deposited: 13 Mar 2014 19:28
Last Modified: 13 Mar 2014 19:28
URI: http://repository.cshl.edu/id/eprint/29659

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