RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans

Piano, F., Schetter, A. J., Mangone, M., Stein, L., Kemphues, K. J. (December 2000) RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans. Current Biology, 10 (24). pp. 1619-1622. ISSN 0960-9822

URL: http://www.ncbi.nlm.nih.gov/pubmed/11137018
DOI: 10.1016/S0960-9822(00)00869-1

Abstract

As a step towards comprehensive functional analysis of genomes, systematic gene knockout projects have been initiated in several organisms [1]. In metazoans like C. elegans, however, maternal contribution can mask the effects of gene knockouts on embryogenesis. RNA interference (RNAi) provides an alternative rapid approach to obtain loss-of-function information that can also reveal embryonic roles for the genes targeted [2,3]. We have used RNAi to analyze a random set of ovarian transcripts and have identified 81 genes with essential roles in embryogenesis, Surprisingly, none of them maps on the X chromosome. Of these 81 genes, 68 showed defects before the eight-cell stage and could be grouped into ten phenotypic classes. To archive and distribute these data we have developed a database system directly linked to the C. elegans database (Wormbase). We conclude that screening cDNA libraries by RNAi is an efficient way of obtaining in vivo function for a large group of genes. Furthermore, this approach is directly applicable to other organisms sensitive to RNAi and whose genomes have not yet been sequenced.

Item Type: Paper
Uncontrolled Keywords: CELL-DIVISION EMBRYOS PROTEIN DATABASES SEQUENCE ENCODES GENOME
Subjects: organism description > animal > C elegans
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Stein lab
Watson School > Publications
Depositing User: Matt Covey
Date: December 2000
Date Deposited: 28 Jan 2014 21:02
Last Modified: 26 Sep 2014 15:13
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29444

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