Piano, F., Schetter, A. J., Mangone, M., Stein, L., Kemphues, K. J. (December 2000) RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans. Current Biology, 10 (24). pp. 1619-1622. ISSN 0960-9822
Abstract
As a step towards comprehensive functional analysis of genomes, systematic gene knockout projects have been initiated in several organisms [1]. In metazoans like C. elegans, however, maternal contribution can mask the effects of gene knockouts on embryogenesis. RNA interference (RNAi) provides an alternative rapid approach to obtain loss-of-function information that can also reveal embryonic roles for the genes targeted [2,3]. We have used RNAi to analyze a random set of ovarian transcripts and have identified 81 genes with essential roles in embryogenesis, Surprisingly, none of them maps on the X chromosome. Of these 81 genes, 68 showed defects before the eight-cell stage and could be grouped into ten phenotypic classes. To archive and distribute these data we have developed a database system directly linked to the C. elegans database (Wormbase). We conclude that screening cDNA libraries by RNAi is an efficient way of obtaining in vivo function for a large group of genes. Furthermore, this approach is directly applicable to other organisms sensitive to RNAi and whose genomes have not yet been sequenced.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CELL-DIVISION EMBRYOS PROTEIN DATABASES SEQUENCE ENCODES GENOME |
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs > Stein lab School of Biological Sciences > Publications |
| Depositing User: | Matt Covey |
| Date: | December 2000 |
| Date Deposited: | 28 Jan 2014 21:02 |
| Last Modified: | 26 Sep 2014 15:13 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29444 |
Actions (login required)
 |
Administrator's edit/view item |