Genetic analysis of chemoresistance in primary murine lymphomas

Schmitt, C. A., Rosenthal, C. T., Lowe, S. W. (September 2000) Genetic analysis of chemoresistance in primary murine lymphomas. Nature Medicine, 6 (9). pp. 1029-1035. ISSN 1078-8956

URL: http://www.ncbi.nlm.nih.gov/pubmed/10973324
DOI: 10.1038/79542

Abstract

Understanding the basis of chemoresistance is a principal goal of molecular oncology. We have exploited a murine lymphoma model and retroviral gene transfer to rapidly generate a series of spontaneous tumors differing only in a gene of interest, and subsequently studied the impact of the test gene on the treatment sensitivity of tumors at their natural site. We demonstrate that the Bcl-2 oncoprotein produces multi-drug resistance when assessed in primary lymphomas in vivo. In contrast, this effect was dramatically reduced when the primary lymphomas were subjected to long-term culture, and completely missed in the standard clonogenic survival assay. This model highlights the importance of physiological test systems to address the complexity of clinical drug resistance and provides a novel strategy to evaluate compounds targeting specific genetic lesions.

Item Type: Paper
Uncontrolled Keywords: NON-HODGKINS-LYMPHOMAS B-CELL C-MYC CLONOGENIC SURVIVAL HEMATOLOGIC MALIGNANCIES ANTICANCER AGENTS INDUCED APOPTOSIS TREATMENT FAILURE TRANSGENIC MICE DRUG-RESISTANCE
Subjects: diseases & disorders > cancer > cancer types > B cell lymphoma
diseases & disorders > cancer
diseases & disorders > cancer > drugs and therapies > chemoresistance
CSHL Authors:
Communities: CSHL labs > Lowe lab
Depositing User: Matt Covey
Date: September 2000
Date Deposited: 30 Jan 2014 22:01
Last Modified: 30 Jan 2014 22:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29392

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