Schmitt, C. A., Rosenthal, C. T., Lowe, S. W. (September 2000) Genetic analysis of chemoresistance in primary murine lymphomas. Nature Medicine, 6 (9). pp. 1029-1035. ISSN 1078-8956
Abstract
Understanding the basis of chemoresistance is a principal goal of molecular oncology. We have exploited a murine lymphoma model and retroviral gene transfer to rapidly generate a series of spontaneous tumors differing only in a gene of interest, and subsequently studied the impact of the test gene on the treatment sensitivity of tumors at their natural site. We demonstrate that the Bcl-2 oncoprotein produces multi-drug resistance when assessed in primary lymphomas in vivo. In contrast, this effect was dramatically reduced when the primary lymphomas were subjected to long-term culture, and completely missed in the standard clonogenic survival assay. This model highlights the importance of physiological test systems to address the complexity of clinical drug resistance and provides a novel strategy to evaluate compounds targeting specific genetic lesions.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | NON-HODGKINS-LYMPHOMAS B-CELL C-MYC CLONOGENIC SURVIVAL HEMATOLOGIC MALIGNANCIES ANTICANCER AGENTS INDUCED APOPTOSIS TREATMENT FAILURE TRANSGENIC MICE DRUG-RESISTANCE |
| Subjects: | diseases & disorders > cancer > cancer types > B cell lymphoma diseases & disorders > cancer diseases & disorders > cancer > drugs and therapies > chemoresistance |
| CSHL Authors: | |
| Communities: | CSHL labs > Lowe lab |
| Depositing User: | Matt Covey |
| Date: | September 2000 |
| Date Deposited: | 30 Jan 2014 22:01 |
| Last Modified: | 30 Jan 2014 22:01 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29392 |
Actions (login required)
 |
Administrator's edit/view item |