Imaging a population code for odor identity in the Drosophila mushroom body

Campbell, R. A., Honegger, K. S., Qin, H., Li, W., Demir, E., Turner, G. C. (June 2013) Imaging a population code for odor identity in the Drosophila mushroom body. Journal of Neuroscience, 33 (25). pp. 10568-81. ISSN 0270-6474

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URL: http://www.ncbi.nlm.nih.gov/pubmed/23785169
DOI: 10.1523/jneurosci.0682-12.2013

Abstract

The brain represents sensory information in the coordinated activity of neuronal ensembles. Although the microcircuits underlying olfactory processing are well characterized in Drosophila, no studies to date have examined the encoding of odor identity by populations of neurons and related it to the odor specificity of olfactory behavior. Here we used two-photon Ca(2+) imaging to record odor-evoked responses from >100 neurons simultaneously in the Drosophila mushroom body (MB). For the first time, we demonstrate quantitatively that MB population responses contain substantial information on odor identity. Using a series of increasingly similar odor blends, we identified conditions in which odor discrimination is difficult behaviorally. We found that MB ensemble responses accounted well for olfactory acuity in this task. Kenyon cell ensembles with as few as 25 cells were sufficient to match behavioral discrimination accuracy. Using a generalization task, we demonstrated that the MB population code could predict the flies' responses to novel odors. The degree to which flies generalized a learned aversive association to unfamiliar test odors depended upon the relative similarity between the odors' evoked MB activity patterns. Discrimination and generalization place different demands on the animal, yet the flies' choices in these tasks were reliably predicted based on the amount of overlap between MB activity patterns. Therefore, these different behaviors can be understood in the context of a single physiological framework.

Item Type: Paper
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body
organism description > animal behavior > odor recognition
organs, tissues, organelles, cell types and functions
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs > Kepecs lab
CSHL labs > Turner lab
Watson School > Publications
Depositing User: Matt Covey
Date: 19 June 2013
Date Deposited: 27 Jun 2013 20:50
Last Modified: 25 Oct 2018 16:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28391

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