Eukaryotic Argonautes come into focus

Kuhn, C. D., Joshua-Tor, L. (March 2013) Eukaryotic Argonautes come into focus. Trends in Biochemical Sciences, 38 (5). pp. 263-271. ISSN 09680004 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/23541793

DOI: 10.1016/j.tibs.2013.02.008

Abstract

Despite the fact that different classes of small RNAs are generated by largely different biogenesis pathways, all mature small RNAs associate with an Argonaute family member to form the RNA-induced silencing complex (RISC). Gene silencing by RISC could not be studied in molecular detail because structural information on eukaryotic Argonautes was lacking. Recently, however, the structure of human Argonaute-2 (hAgo2), a model for RISC function, was determined in complexes with heterogeneous guide RNA and in complexes with a specific miRNA. We review the exciting advances that these two structures, together with the structure of a budding yeast Argonaute, brought to the field of eukaryotic RNA interference (RNAi), and how they will enable a more detailed mechanistic understanding of eukaryotic RISC.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types > eukaryote organs, tissues, organelles, cell types and functions > cell types and functions > cell types > eukaryote organs, tissues, organelles, cell types and functions > cell types and functions > cell types > eukaryote bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:	Joshua-Tor, Leemor Kuhn, Claus-D.
Communities:	CSHL labs > Joshua-Tor lab
Depositing User:	Matt Covey
Date:	28 March 2013
Date Deposited:	23 May 2013 14:32
Last Modified:	23 May 2013 14:32
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28330

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