Persistent activation of the ζ isoform of protein kinase C in the maintenance of long-term potentiation

Sacktor, T. C., Osten, P., Valsamis, H., Jiang, X., Naik, M. U., Sublette, E. (1993) Persistent activation of the ζ isoform of protein kinase C in the maintenance of long-term potentiation. Proceedings of the National Academy of Sciences of the United States of America, 90 (18). pp. 8342-8346. ISSN 00278424 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8378304

Abstract

Long-term potentiation in the CA1 region of the hippocampus, a model for memory formation in the brain, is divided into two phases. A transient process (induction) is initiated, which then generates a persistent mechanism (maintenance) for enhancing synaptic strength. Protein kinase C (PKC), a gene family of multiple isozymes, may play a role in both induction and maintenance. In region CA1 from rat hippocampal slices, most of the isozymes of PKC translocated to the particulate fraction 15 sec after a tetanus. The increase of PKC in the particulate fraction did not persist into the maintenance phase of long-term potentiation. In contrast, a constitutively active kinase, PKM, a form specific to a single isozyme (ζ), increased in the cytosol during the maintenance phase. The transition from translocation of PKC to formation of PKM may help to explain the molecular mechanisms of induction and maintenance of long-term potentiation.

Item Type: Paper
Uncontrolled Keywords: isoenzyme n methyl dextro aspartic acid receptor protein kinase c animal tissue article autophosphorylation brain slice controlled study enzyme activation excitatory postsynaptic potential hippocampus immunoblotting long term potentiation memory nonhuman peptide mapping priority journal rat Amino Acid Sequence Animal Electric Stimulation Evoked Potentials In Vitro Isoenzymes Kinetics Models, Neurological Molecular Sequence Data Oligopeptides Peptide Fragments Phosphorylation Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate Support, U.S. Gov't, P.H.S. Time Factors
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs > Osten lab
Depositing User: Matt Covey
Date: 1993
Date Deposited: 05 Apr 2013 02:15
Last Modified: 05 Apr 2013 02:15
PMCID: PMC47352
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28090

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