Sacktor, T. C., Osten, P., Valsamis, H., Jiang, X., Naik, M. U., Sublette, E.
(1993)
Persistent activation of the ζ isoform of protein kinase C in the maintenance of long-term potentiation.
Proceedings of the National Academy of Sciences of the United States of America, 90 (18).
pp. 8342-8346.
ISSN 00278424 (ISSN)
Abstract
Long-term potentiation in the CA1 region of the hippocampus, a model for memory formation in the brain, is divided into two phases. A transient process (induction) is initiated, which then generates a persistent mechanism (maintenance) for enhancing synaptic strength. Protein kinase C (PKC), a gene family of multiple isozymes, may play a role in both induction and maintenance. In region CA1 from rat hippocampal slices, most of the isozymes of PKC translocated to the particulate fraction 15 sec after a tetanus. The increase of PKC in the particulate fraction did not persist into the maintenance phase of long-term potentiation. In contrast, a constitutively active kinase, PKM, a form specific to a single isozyme (ζ), increased in the cytosol during the maintenance phase. The transition from translocation of PKC to formation of PKM may help to explain the molecular mechanisms of induction and maintenance of long-term potentiation.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
isoenzyme
n methyl dextro aspartic acid receptor
protein kinase c
animal tissue
article
autophosphorylation
brain slice
controlled study
enzyme activation
excitatory postsynaptic potential
hippocampus
immunoblotting
long term potentiation
memory
nonhuman
peptide mapping
priority journal
rat
Amino Acid Sequence
Animal
Electric Stimulation
Evoked Potentials
In Vitro
Isoenzymes
Kinetics
Models, Neurological
Molecular Sequence Data
Oligopeptides
Peptide Fragments
Phosphorylation
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate
Support, U.S. Gov't, P.H.S.
Time Factors |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > tissues types and functions |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Osten lab |
| Depositing User: |
Matt Covey
|
| Date: |
1993 |
| Date Deposited: |
05 Apr 2013 02:15 |
| Last Modified: |
13 Sep 2019 17:03 |
| PMCID: |
PMC47352 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28090 |
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