Conditioning deficits of CaM-kinase transgenic Drosophila melanogaster in a new excitatory courtship assay

Broughton, S. J., Tully, T., Greenspan, R. J. (January 2003) Conditioning deficits of CaM-kinase transgenic Drosophila melanogaster in a new excitatory courtship assay. Journal of Neurogenetics, 17 (1). pp. 91-102. ISSN 0167-7063

URL: http://www.ncbi.nlm.nih.gov/pubmed/14504030
DOI: 10.1080/01677060390228342

Abstract

Courtship suppression is an associative conditioning procedure in Drosophila melanogaster that is ethologically based and capable of being tested on individual flies. We have expanded the range of the courtship conditioning by developing an excitatory procedure in which male flies learn to associate a novel odor with the courtship stimulating cues of virgin females. Wild-type males normally court other mature males very little, but following training, the odor alone is able to elicit increased levels of courtship towards an object male. Flies expressing an inhibitor of calcium/calmodulin-dependent protein kinase II (CaMKII) were previously shown to have no retention one hour after training in the courtship suppression task, as manifested in their persistent courting of a virgin female. A possible trivial explanation for this response is that the CaMKII-inhibited fly strains (ala1 and ala2) were merely hyperactive courters. The poor performance of these mutants in the new excitatory conditioning procedure confirms that their conditioning deficit results from a disruption of an associative mechanism per se.

Item Type: Paper
Uncontrolled Keywords: male-specific behavior modified courtship calcium-calmodulin-dependent protein kinase II transgenic disruption of CaM-kinase II activity DEPENDENT PROTEIN-KINASE MUTANT DROSOPHILA BEHAVIOR RESPONSES MEMORY ACQUISITION PLASTICITY MUTATIONS RETENTION APLYSIA
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > animal behavior
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Tully lab
Depositing User: Matt Covey
Date: January 2003
Date Deposited: 01 Apr 2013 19:37
Last Modified: 01 Apr 2013 19:37
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27984

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