Differential expression of miRNAs in response to salt stress in maize roots

Ding, D., Zhang, L., Wang, H., Liu, Z., Zhang, Z., Zheng, Y. (2009) Differential expression of miRNAs in response to salt stress in maize roots. Ann Bot, 103 (1). pp. 29-38.

URL: http://www.ncbi.nlm.nih.gov/pubmed/18952624
DOI: 10.1093/aob/mcn205

Abstract

Background and AimsCorn (Zea mays) responds to salt stress via changes in gene expression, metabolism and physiology. This adaptation is achieved through the regulation of gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) have been found to act as key regulating factors of post-transcriptional gene expression. However, little is known about the role of miRNAs in plants' responses to abiotic stresses. MethodsA custom {micro}parafloTM microfluidic array containing release version 10.1 plant miRNA probes (http://microrna.sanger.ac.uk/) was used to discover salt stress-responsive miRNAs using the differences in miRNA expression between the salt-tolerant maize inbred line NC286' and the salt-sensitive maize line Huangzao4'. Key ResultsmiRNA microarray hybridization revealed that a total of 98 miRNAs, from 27 plant miRNA families, had significantly altered expression after salt treatment. These miRNAs displayed different activities in the salt response, and miRNAs belonging to the same miRNA family showed the same behaviour. Interestingly, 18 miRNAs were found which were only expressed in the salt-tolerant maize line, and 25 miRNAs that showed a delayed regulation pattern in the salt-sensitive line. A gene model was proposed that showed how miRNAs could regulate the abiotic stress-associated process and the gene networks coping with the stress. ConclusionsSalt-responsive miRNAs are involved in the regulation of metabolic, morphological and physiological adaptations of maize seedlings at the post-transcriptional level. The miRNA genotype-specific expression model might explain the distinct salt sensitivities between maize lines.

Item Type: Paper
Uncontrolled Keywords: Salt stress Zea mays microRNA microarray transcription regulation Zea mays
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organism description > plant
CSHL Authors:
Communities: CSHL labs > Ware lab
Depositing User: Matt Covey
Date Deposited: 21 Feb 2013 17:42
Last Modified: 21 Feb 2013 17:42
PMCID: PMC2707283
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27362

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