Mutations in an AP2 transcription factor-like gene affect internode length and leaf shape in maize

Jiang, F., Guo, M., Yang, F., Duncan, K., Jackson, D., Rafalski, A., Wang, S., Li, B. (May 2012) Mutations in an AP2 transcription factor-like gene affect internode length and leaf shape in maize. PLoS ONE, 7 (5). Article no.e37040.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/22649507
DOI: 0.1371/journal.pone.0037040

Abstract

Background Plant height is an important agronomic trait that affects yield and tolerance to certain abiotic stresses. Understanding the genetic control of plant height is important for elucidating the regulation of maize development and has practical implications for trait improvement in plant breeding. Methodology/Principal Findings In this study, two independent, semi-dwarf maize EMS mutants, referred to as dwarf & irregular leaf (dil1), were isolated and confirmed to be allelic. In comparison to wild type plants, the mutant plants have shorter internodes, shorter, wider and wrinkled leaves, as well as smaller leaf angles. Cytological analysis indicated that the leaf epidermal cells and internode parenchyma cells are irregular in shape and are arranged in a more random fashion, and the mutants have disrupted leaf epidermal patterning. In addition, parenchyma cells in the dil1 mutants are significantly smaller than those in wild-type plants. The dil1 mutation was mapped on the long arm of chromosome 6 and a candidate gene, annotated as an AP2 transcription factor-like, was identified through positional cloning. Point mutations near exon-intron junctions were identified in both dil1 alleles, resulting in mis-spliced variants. Conclusion An AP2 transcription factor-like gene involved in stalk and leaf development in maize has been identified. Mutations near exon-intron junctions of the AP2 gene give mis-spliced transcript variants, which result in shorter internodes and wrinkled leaves.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Jackson lab
CSHL Post Doctoral Fellows
Depositing User: Matt Covey
Date: 23 May 2012
Date Deposited: 30 Jan 2013 19:21
Last Modified: 03 May 2013 15:54
PMCID: PMC3359370
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26972

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