Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system

Maroney, P. A., Hannon, G. J., Nilsen, T. W. (1990) Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system. Proceedings of the National Academy of Sciences of the United States of America, 87 (2). pp. 709-713. ISSN 00278424 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2300557

Abstract

Maturation of a fraction of mRNAs in nematodes involves the acquisition of a common 5' terminal spliced leader sequence derived from a nonpolyadenylated spliced leader RNA by trans splicing. We have developed a cell-free system prepared from Ascaris lumbricoides embryos that accurately and efficiently synthesized the spliced leader RNA of A. lumbricoides. Transcription of the spliced leader RNA was catalyzed by RNA polymerase II, and the majority of the spliced leader RNAs synthesized in vitro possessed a trimethylguanosine cap structure identical to that found on in vivo-synthesized spliced leader RNA.

Item Type: Paper
Uncontrolled Keywords: capping RNA polymerase II small nuclear RNAs rna polymerase small nuclear rna article dna transcription nematode nonhuman priority journal rna splicing Animal Ascaris Base Sequence Cell-Free System Embryo, Nonmammalian Methylation Molecular Sequence Data Nucleotide Mapping RNA Caps RNA, Messenger RNA, Small Nuclear Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Transcription, Genetic Ascaris lumbricoides Nematoda
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date Deposited: 09 Jan 2013 17:35
Last Modified: 09 Jan 2013 17:35
PMCID: PMC53335
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26501

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