Maroney, P. A., Hannon, G. J., Nilsen, T. W. (1990) Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system. Proceedings of the National Academy of Sciences of the United States of America, 87 (2). pp. 709-713. ISSN 00278424 (ISSN)
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Abstract
Maturation of a fraction of mRNAs in nematodes involves the acquisition of a common 5' terminal spliced leader sequence derived from a nonpolyadenylated spliced leader RNA by trans splicing. We have developed a cell-free system prepared from Ascaris lumbricoides embryos that accurately and efficiently synthesized the spliced leader RNA of A. lumbricoides. Transcription of the spliced leader RNA was catalyzed by RNA polymerase II, and the majority of the spliced leader RNAs synthesized in vitro possessed a trimethylguanosine cap structure identical to that found on in vivo-synthesized spliced leader RNA.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | capping RNA polymerase II small nuclear RNAs rna polymerase small nuclear rna article dna transcription nematode nonhuman priority journal rna splicing Animal Ascaris Base Sequence Cell-Free System Embryo, Nonmammalian Methylation Molecular Sequence Data Nucleotide Mapping RNA Caps RNA, Messenger RNA, Small Nuclear Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Transcription, Genetic Ascaris lumbricoides Nematoda |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | 1990 |
| Date Deposited: | 09 Jan 2013 17:35 |
| Last Modified: | 30 Sep 2019 16:36 |
| PMCID: | PMC53335 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26501 |
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