Transcription of a nematode U1 small nuclear RNA in vitro. 3'-end formation requires cis-acting elements within the coding sequence

Hannon, G. E., Hannon, G. J., Maroney, P. A., Nilsen, T. W. (1994) Transcription of a nematode U1 small nuclear RNA in vitro. 3'-end formation requires cis-acting elements within the coding sequence. Journal of Biological Chemistry, 269 (17). pp. 12387-12390. ISSN 00219258 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8175641

Abstract

We have used block-substitution mutagenesis and in vitro transcription assays to identify cis-acting DNA sequence elements important for initiation and 3'-end formation of a U1 small nuclear RNA (snRNA) in the parasitic nematode Ascaris lumbricoides. Efficient initiation of synthesis by RNA polymerase II requires a compact element centered ~50 base pairs upstream of the transcriptional start site. Surprisingly, 3'-end formation of U1 snRNAs synthesized in vitro is solely dependent upon elements within the U1 coding sequence. In all other systems studied thus far, 3'-end formation of U snRNAs requires signals present in the 3'-flanking region. We also show that sequence elements that direct 3'-end formation of the A. lumbricoides trans- spliced leader RNA function when RNA synthesis is initiated from the U1 promoter. These results indicate that 3'-end formation of U snRNAs in nematodes is mechanistically distinct from the analogous process in higher eukaryotes.

Item Type: Paper
Uncontrolled Keywords: rna polymerase ii small nuclear rna article ascaris lumbricoides base pairing dna flanking region dna sequence dna template enzyme mechanism nonhuman phylogeny priority journal promoter region rna splicing site directed mutagenesis transcription initiation Animal Base Sequence DNA Molecular Sequence Data Mutagenesis, Site-Directed Regulatory Sequences, Nucleic Acid RNA, Small Nuclear Support, U.S. Gov't, P.H.S. Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA regulation
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1994
Date Deposited: 09 Jan 2013 17:37
Last Modified: 09 Jan 2013 17:37
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26492

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