Shutdown is a component of the Drosophila piRNA biogenesis machinery

Preall, J. B., Czech, B., Guzzardo, P. M., Muerdter, F., Hannon, G. J. (2012) Shutdown is a component of the Drosophila piRNA biogenesis machinery. RNA, 18 (8). pp. 1446-1457. ISSN 13558382 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/22753781
DOI: 10.1261/rna.034405.112

Abstract

In animals, the piRNA pathway preserves the integrity of gametic genomes, guarding them against the activity of mobile genetic elements. This innate immune mechanism relies on distinct genomic loci, termed piRNA clusters, to provide a molecular definition of transposons, enabling their discrimination from genes. piRNA clusters give rise to long, single-stranded precursors, which are processed into primary piRNAs through an unknown mechanism. These can engage in an adaptive amplification loop, the ping-pong cycle, to optimize the content of small RNA populations via the generation of secondary piRNAs.Many proteins have been ascribed functions in either primary biogenesis or the ping-pong cycle, though for the most part the molecular functions of proteins implicated in these pathways remain obscure. Here, we link shutdown (shu), a gene previously shown to be required for fertility in Drosophila, to the piRNA pathway. Analysis of knockdown phenotypes in both the germline and somatic compartments of the ovary demonstrate important roles for shutdown in both primary biogenesis and the ping-pong cycle. shutdown is a member of the FKBP family of immunophilins. Shu contains domains implicated in peptidyl-prolyl cis-trans isomerase activity and in the binding of HSP90-family chaperones, though the relevance of these domains to piRNA biogenesis is unknown. Published by Cold Spring Harbor Laboratory Press. Copyright © 2012 RNA Society.

Item Type: Paper
Uncontrolled Keywords: FKBP Germ cells piRNAs RNAi Transposon silencing chaperone fk 506 binding protein heat shock protein 90 immunophilin peptidylprolyl isomerase Piwi interacting RNA Piwi protein protein protein Ago3 protein Aubergine unclassified drug animal experiment animal tissue article biogenesis controlled study Drosophila enzyme activity female fertility gene gene silencing germ line male nonhuman nucleotide sequence ovary phenotype priority journal protein binding protein domain protein function RNA biogenesis shutdown gene transposon Animals Drosophila melanogaster Drosophila Proteins Fluorescent Antibody Technique Real-Time Polymerase Chain Reaction Retroelements Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Small Interfering Tacrolimus Binding Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Hannon lab
Watson School > Publications
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
Depositing User: Matt Covey
Date: 2012
Date Deposited: 09 Jan 2013 16:12
Last Modified: 09 Nov 2017 21:23
PMCID: PMC3404366
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26414

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