Fluctuation analysis of motor protein movement and single enzyme kinetics

Svoboda, K., Mitra, P. P., Block, S. M. (December 1994) Fluctuation analysis of motor protein movement and single enzyme kinetics. Proc Natl Acad Sci U S A, 91 (25). pp. 11782-6. ISSN 0027-8424

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URL: https://www.ncbi.nlm.nih.gov/pubmed/7991536

Abstract

We studied fluctuations in the displacement of silica beads driven by single molecules of the motor protein kinesin, moving under low mechanical loads at saturating ATP concentrations. The variance in position was significantly smaller than expected for the case of stepwise movement along a regular lattice of positions with exponentially distributed intervals. The small variance suggests that two or more sequential processes with comparable reaction rates dominate the biochemical cycle. The low value is inconsistent with certain recently proposed thermal ratchet models for motor movement as well as with scenarios where the hydrolysis of a single ATP molecule leads to a cluster of several steps. Fluctuation analysis is a potential powerful tool for studying kinetic behavior whenever the output of a single enzyme can be monitored.

Item Type: Paper
Uncontrolled Keywords: Animals Decapodiformes Kinesin chemistry isolation & purification metabolism Kinetics Mathematics Nervous System enzymology Probability Time Factors
Subjects: physics > biophysics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
physics
CSHL Authors:
Communities: CSHL labs > Mitra lab
Depositing User: CSHL Librarian
Date: 6 December 1994
Date Deposited: 02 Apr 2012 20:22
Last Modified: 09 Nov 2017 19:35
PMCID: PMC45319
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25886

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