Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin

Tsurimoto, T., Melendy, T., Stillman, B. (1990) Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin. Nature, 346 (6284). pp. 534-9. ISSN 0028-0836

URL: http://www.ncbi.nlm.nih.gov/pubmed/2165567
DOI: 10.1038/346534a0

Abstract

Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase alpha-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase delta complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase delta complex. A model for polymerase switching during initiation of DNA replication is presented.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Animals Cattle Cell Nucleus metabolism DNA Polymerase III DNA Primase DNA Replication DNA-Directed DNA Polymerase metabolism Escherichia coli genetics Humans Kinetics Models Genetic RNA Nucleotidyltransferases metabolism Simian virus 40 genetics Thymus Gland enzymology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 28 Feb 2012 19:34
Last Modified: 20 Jun 2017 20:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25116

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving