Tsurimoto, T., Melendy, T., Stillman, B. (August 1990) Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin. Nature, 346 (6284). pp. 534-9. ISSN 0028-0836
Abstract
Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase alpha-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase delta complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase delta complex. A model for polymerase switching during initiation of DNA replication is presented.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | Animals Cattle Cell Nucleus metabolism DNA Polymerase III DNA Primase DNA Replication DNA-Directed DNA Polymerase metabolism Escherichia coli genetics Humans Kinetics Models Genetic RNA Nucleotidyltransferases metabolism Simian virus 40 genetics Thymus Gland enzymology |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 9 August 1990 |
| Date Deposited: | 28 Feb 2012 19:34 |
| Last Modified: | 20 Jun 2017 20:14 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25116 |
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