Identification of Nuclear Dicing Bodies Containing Proteins for MicroRNA Biogenesis in Living Arabidopsis Plants

Fang, Y., Spector, D. L. (2007) Identification of Nuclear Dicing Bodies Containing Proteins for MicroRNA Biogenesis in Living Arabidopsis Plants. Current Biology, 17 (9). pp. 818-823. ISSN 0960-9822 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17442570
DOI: 10.1016/j.cub.2007.04.005

Abstract

MicroRNAs (miRNAs) are important for regulating gene expression in muticellular organisms. MiRNA processing is a two-step process. In animal cells, the first step is nuclear and the second step cytoplasmic, whereas in plant cells, both steps occur in the nucleus via the enzyme Dicer-like1 (DCL1) [1, 2] and other proteins including the zinc-finger-domain protein Serrate (SE) [3, 4] and a double-stranded RNA (dsRNA) binding-domain protein, Hyponastic Leaves1 (HYL1) [5-7]. Furthermore, plant miRNAs are methylated by Hua Enhancer (HEN1) at their 3' ends [8] and loaded onto Argonaute1 (AGO1) [9]. However, little is known about the cellular basis of miRNA biogenesis. Using live-cell imaging, we show here that DCL1 and HYL1 colocalize in discrete nuclear bodies in addition to being present in a low-level diffuse nucleoplasmic distribution. These bodies, which we refer to as nuclear dicing bodies (D-bodies), differ from Cajal bodies [10, 11]. A mutated DCL1 with impaired function in miRNA processing fails to target to D-bodies, and an introduced primary (pri)-miRNA transcript is recruited to D-bodies. Furthermore, bimolecular fluorescence complementation (BiFC) shows that DCL1, HYL1, and SE interact in D-bodies. On the basis of these data, we propose that D-bodies are crucial for orchestrating pri-miRNA processing and/or storage/assembly of miRNA-processing complexes in the nuclei of plant cells.

Item Type: Paper
Additional Information: Journal article
Uncontrolled Keywords: TRANS-ACTING SIRNAS CAJAL BODIES PROCESSING CENTER GENE-EXPRESSION THALIANA HYL1 PATHWAY DCL4 METHYLATION SERRATE RNA
Subjects: organism description > plant > Arabidopsis
organism description > plant behavior
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > dicer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > dicer

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > zinc finger proteins
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: CSHL Librarian
Date Deposited: 15 Nov 2011 17:32
Last Modified: 28 Jan 2015 21:09
PMCID: PMC1950788
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23014

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