Abortive initiation and productive initiation by RNA polymerase involve DNA scrunching

Revyakin, A., Liu, C., Ebright, R. H., Strick, T. R. (November 2006) Abortive initiation and productive initiation by RNA polymerase involve DNA scrunching. Science, 314 (5802). pp. 1139-1143. ISSN 00368075

URL: https://www.ncbi.nlm.nih.gov/pubmed/17110577
DOI: 10.1126/science.1131398

Abstract

Using single-molecule DNA nanomanipulation, we show that abortive initiation involves DNA "scrunching"-in which RNA polymerase (RNAP) remains stationary and unwinds and pulls downstream DNA into itself-and that scrunching requires RNA synthesis and depends on RNA length. We show further that promoter escape involves scrunching, and that scrunching occurs in most or all instances of promoter escape. Our results support the existence of an obligatory stressed intermediate, with approximately one turn of additional DNA unwinding, in escape and are consistent with the proposal that stress in this intermediate provides the driving force to break RNAP-promoter and RNAP-initiation-factor interactions in escape.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
CSHL Authors:
Communities: CSHL labs > Strick lab
Depositing User: CSHL Librarian
Date: 17 November 2006
Date Deposited: 12 Dec 2011 16:08
Last Modified: 27 Apr 2018 14:58
PMCID: PMC2754787
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22884

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