Revyakin, A., Liu, C., Ebright, R. H., Strick, T. R. (November 2006) Abortive initiation and productive initiation by RNA polymerase involve DNA scrunching. Science, 314 (5802). pp. 1139-1143. ISSN 00368075
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Abstract
Using single-molecule DNA nanomanipulation, we show that abortive initiation involves DNA "scrunching"-in which RNA polymerase (RNAP) remains stationary and unwinds and pulls downstream DNA into itself-and that scrunching requires RNA synthesis and depends on RNA length. We show further that promoter escape involves scrunching, and that scrunching occurs in most or all instances of promoter escape. Our results support the existence of an obligatory stressed intermediate, with approximately one turn of additional DNA unwinding, in escape and are consistent with the proposal that stress in this intermediate provides the driving force to break RNAP-promoter and RNAP-initiation-factor interactions in escape.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase |
| CSHL Authors: | |
| Communities: | CSHL labs > Strick lab |
| Depositing User: | CSHL Librarian |
| Date: | 17 November 2006 |
| Date Deposited: | 12 Dec 2011 16:08 |
| Last Modified: | 03 Oct 2019 13:49 |
| PMCID: | PMC2754787 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22884 |
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