Regulation of adeno-associated virus DNA replication by the cellular TAF-I/set complex

Pegoraro, G., Marcello, A., Myers, M. P., Giacca, M. (July 2006) Regulation of adeno-associated virus DNA replication by the cellular TAF-I/set complex. Journal of Virology, 80 (14). pp. 6855-6864. ISSN 0022538X

URL: https://www.ncbi.nlm.nih.gov/pubmed/16809291
DOI: 10.1128/JVI.00383-06

Abstract

The Rep proteins of the adeno-associated virus (AAV) are required for viral replication in the presence of adenovirus helper functions and as yet poorly characterized cellular factors. In an attempt to identify such factors, we purified Flag-Rep68-interacting proteins from human cell lysates. Several polypeptides were identified by mass spectrometry, among which was ANP32B, a member of the acidic nuclear protein 32 family which takes part in the formation of the template-activating factor I/Set oncoprotein (TAF-I/Set) complex. The N terminus of Rep was found to specifically bind the acidic domain of ANP32B; through this interaction, Rep was also able to recruit other members of the TAF-I/Set complex, including the ANP32A protein and the histone chaperone TAF-I/Set. Further experiments revealed that silencing of ANP32A and ANP32B inhibited AAV replication, while overexpression of all of the components of the TAF-I/Set complex increased de novo AAV DNA synthesis in permissive cells. Besides being the first indication that the TAF-I/Set complex participates in wild-type AAV replication, these findings have important implications for the generation of recombinant AAV vectors since overexpression of the TAF-I/Set components was found to markedly increase viral vector production. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus > adeno associated virus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > polypeptides
CSHL Authors:
Communities: CSHL labs > Myers lab
Depositing User: CSHL Librarian
Date: July 2006
Date Deposited: 09 Dec 2011 18:15
Last Modified: 27 Apr 2018 14:31
PMCID: PMC1489034
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22876

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