Biochemical specialization within Arabidopsis RNA silencing pathways

Qi, Yijun, Denli, Ahmet M., Hannon, Gregory J. (August 2005) Biochemical specialization within Arabidopsis RNA silencing pathways. Mol Cell, 19 (3). pp. 421-8. ISSN 1097-2765 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/16061187
DOI: 10.1016/j.molcel.2005.06.014

Abstract

In plants, the RNA silencing machinery responds to numerous inputs, including viral infection, microRNAs, and endogenous siRNAs that may act both in trans and in cis. Additionally, the full spectrum of silencing outcomes has been demonstrated in plants, ranging from mRNA degradation to repression at the level of protein synthesis to chromatin remodeling. Genetic studies in Arabidopsis have indicated that individual response pathways are functionally compartmentalized. However, to date, no biochemical systems have been available to investigate the roles of specific proteins within silencing pathways or the effects of selected mutations on the biochemical activity of those components. Here, we describe the generation of Arabidopsis extracts that reproduce many aspects of RNA silencing reactions in vitro. We find that specific members of the Dicer and Argonaute families have distinct biochemical activities, which provides insight into their roles within RNA silencing pathways in Arabidopsis.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphate metabolism Arabidopsis genetics metabolism Arabidopsis Proteins genetics metabolism Cell Cycle Proteins genetics metabolism MicroRNAs genetics metabolism Mutation Plant Extracts metabolism RNA Interference RNA Double-Stranded genetics metabolism RNA Messenger genetics metabolism RNA, Small Interfering genetics metabolism RNA Induced Silencing Complex genetics metabolism Ribonuclease III genetics metabolism Signal Transduction genetics
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA silencing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: 5 August 2005
Date Deposited: 06 Jan 2012 14:55
Last Modified: 19 Sep 2014 15:51
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22681

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