Two novel proteins, Dos1 and Dos2, interact with Rik1 to regulate heterochromatic RNA interference and histone modification

Li, F., Goto, D. B., Zaratiegui, M., Tang, X., Martienssen, R., Cande, W. Z. (August 2005) Two novel proteins, Dos1 and Dos2, interact with Rik1 to regulate heterochromatic RNA interference and histone modification. Current Biology, 15 (16). pp. 1448-1457. ISSN 0960-9822

URL: http://www.ncbi.nlm.nih.gov/pubmed/16040243
DOI: 10.1016/j.cub.2005.07.021

Abstract

Background: Chromosomal behavior during mitosis and meiosis depends in part on heterochromatic modifications such as histone H3 lysine-9 methylation (H3K9me). In fission yeast, the Heterochromatin Protein 1 homolog Swi6 recognizes H3K9me, silences transcription, and retains cohesin at pericentromeric repeats. Heterochromatin formation also depends on processing of transcripts derived from centromeric repeats by the RNAi machinery. The DDB1 homolog, Rik1, and histone methyltransferase, CIr4, act in a complex to promote H3K9me. However, the mechanism underlying this interaction is poorly understood. Results: Using a cytological screen, we have identified two novel genes, dos1(+) and dos2(+), which are required for localization of Swi6. Deletion of either of these genes results in mitotic and meiotic chromosome missegregation, defects in mitotic centromeric cohesion and meiotic telomere clustering, and loss of heterochromatic silencing. Dos1 is predominantly located in the nucleus in a Dos2-dependent manner and directly interacts with Rik1. Each of these genes is required for the association of H3K9me with centromeric repeats, as well as for the production of small interfering RNAs. Conclusions: Dosl and Dos2 are required for the formation of heterochromatin in fission yeast. We hypothesize that the physical interaction between Dosl and Rik1 represents a role in regulating activity of the Rik1/ CIr4 complex. Dos2 contributes to this role by regulating Dosl localization. Our findings suggest a mechanism for recruitment of CIr4 in the RNAi-dependent heterochromatin pathway, in which Dos1 and Dos2 are essential.

Item Type: Paper
Uncontrolled Keywords: disrupt chromosome segregation fission yeast centromeres H3 Lysine-9 Lysine 9 methylation schizosaccharomyces-pompe Schizosaccharomyces-Pompe GENE meiosis inheritance repeats RITS
Subjects: bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: CSHL Librarian
Date: August 2005
Date Deposited: 09 Jan 2012 19:34
Last Modified: 09 Apr 2014 21:02
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22630

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