Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging

Wang, Y. L., Guo, H. F., Pologruto, T. A., Hannan, F., Hakker, I., Svoboda, K., Zhong, Y. (2004) Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging. Journal of Neuroscience, 24 (29). pp. 6507-6514. ISSN 0270-6474

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URL: http://www.jneurosci.org/content/24/29/6507.full
DOI: 10.1523/​JNEUROSCI.3727-03.2004

Abstract

To study the representation of olfactory information in higher brain centers, we expressed a green fluorescent protein-based Ca2+ sensor, G-CaMP, in the Drosophila mushroom body (MB). Using two-photon microscopy, we imaged odor-evoked G-CaMP fluorescence transients in MB neurons [Kenyon cells (KCs)] with single-cell resolution. Odors produced large fluorescence transients in a subset of KC somata and in restricted regions of the calyx, the neuropil of the MB. In different KCs, odor-evoked fluorescence transients showed diverse changes with odor concentration: in some KCs, fluorescence transients were evoked by an odor at concentrations spanning several orders of magnitude, whereas in others only at a narrow concentration range. Different odors produced fluorescence transients in different subsets of KCs. The spatial distributions of KCs showing fluorescence transients evoked by a given odor were similar across individuals. For some odors, individual KCs with fluorescence transients evoked by a particular odor could be found in similar locations in different flies with spatial precisions on the order of the size of KC somata. These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB.

Item Type: Paper
Uncontrolled Keywords: odor stereotype mushroom body calcium imaging G-CaMP two photon Kenyon cell NICOTINIC ACETYLCHOLINE-RECEPTORS nicotinoc acetylcholine receptors OLFACTORY SENSORY MAP olfactory sensory map PYRIFORM priform CORTEX GENE-EXPRESSION gene expression APIS-MELLIFERA apis mellifera ANTENNAL LOBE antennal lobe KENYON CELLS kenyon cells FLY BRAIN fly brain IN-VIV in-vivo NEURONS neorons
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body
CSHL Authors:
Depositing User: CSHL Librarian
Date Deposited: 17 Jan 2012 17:37
Last Modified: 17 Jan 2012 17:37
URI: http://repository.cshl.edu/id/eprint/22512

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