Gene and enhancer trap tagging of vascular-expressed genes in poplar trees

Groover, A., Fontana, J. R., Dupper, G., Ma, C. P., Martienssen, R. A., Strauss, S., Meilan, R. (April 2004) Gene and enhancer trap tagging of vascular-expressed genes in poplar trees. Plant Physiology, 134 (4). pp. 1742-1751. ISSN 0032-0889

URL: http://www.ncbi.nlm.nih.gov/pubmed/15051863
DOI: 10.1104/pp.103.034330

Abstract

We report a gene discovery system for poplar trees based on gene and enhancer traps. Gene and enhancer trap vectors carrying the beta-glucuronidase (GUS) reporter gene were inserted into the poplar genome via Agrobacterium tumefaciens transformation, where they reveal the expression pattern of genes at or near the insertion sites. Because GUS expression phenotypes are dominant and are scored in primary transformants, this system does not require rounds of sexual recombination, a typical barrier to developmental genetic studies in trees. Gene and enhancer trap lines defining genes expressed during primary and secondary vascular development were identified and characterized. Collectively, the vascular gene expression patterns revealed that approximately 40% of genes expressed in leaves were expressed exclusively in the veins, indicating that a large set of genes is required for vascular development and function. Also, significant overlap was found between the sets of genes responsible for development and function of secondary vascular tissues of stems and primary vascular tissues in other organs of the plant, likely reflecting the common evolutionary origin of these tissues. Chromosomal DNA flanking insertion sites was amplified by thermal asymmetric interlaced PCR and sequenced and used to identify insertion sites by reference to the nascent Populus trichocarpa genome sequence. Extension of the system was demonstrated through isolation of full-length cDNAs for five genes of interest, including a new class of vascular-expressed gene tagged by enhancer trap line cET-1-pop1-145. Poplar gene and enhancer traps provide a new resource that allows plant biologists to directly reference the poplar genome sequence and identify novel genes of interest in forest biology.

Item Type: Paper
Uncontrolled Keywords: PLANT DEVELOPMENT plant development ARABIDOPSIS Arabidopsis POPULUS Populus
Subjects: organism description > plant > Arabidopsis
therapies > viral vectors
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: CSHL Librarian
Date: April 2004
Date Deposited: 03 Feb 2012 17:53
Last Modified: 10 Apr 2014 15:13
PMCID: PMC419847
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22377

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