MicroRNA activity in the Arabidopsis male germline

Borges, F., Pereira, P. A., Slotkin, R. K., Martienssen, R. A., Becker, J. D. (March 2011) MicroRNA activity in the Arabidopsis male germline. Journal of Experimental Botany, 62 (5). pp. 1611-1620. ISSN 0022-0957

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URL: http://www.ncbi.nlm.nih.gov/pubmed/21357774
DOI: 10.1093/jxb/erq452

Abstract

Most of the core proteins involved in the microRNA (miRNA) pathway in plants have been identified, and almost simultaneously hundreds of miRNA sequences processed in the Arabidopsis sporophyte have been discovered by exploiting next-generation sequencing technologies. However, there is very limited understanding about potentially distinct mechanisms of post-transcriptional regulation between different cell lineages. In this review the focus is on the Arabidopsis male gametophyte (pollen), where the germline differentiates after meiosis giving rise to the male gametes. Based on comparative analysis of miRNAs identified in sperm cells by in-depth sequencing, their possible functions during germ cell specification and beyond fertilization are discussed. In addition, 25 potentially novel miRNAs processed in sperm cells and pollen were identified, as well as enriched variations in the sequence length of known miRNAs, which might indicate subfunctionalization by association with a putative germline-specific Argonaute complex. ARGONAUTE 5 (AGO5), by close homology to AGO1 and localizing preferentially to the sperm cell cytoplasm in mature pollen, may be part of such a complex.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis male germ cells microRNA pollen post-transcriptional gene silencing male gametophyte development small rna pathway sirna biogenesis messenger-rna spermatozoal rnas flowering plants argonaute family mirna expression pollen
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > post transcriptional modification
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: CSHL Librarian
Date: March 2011
Date Deposited: 20 Oct 2011 15:06
Last Modified: 09 Apr 2014 19:15
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15607

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