Analysis of the Trajectory of Drosophila melanogaster in a Circular Open Field Arena

Valente, D., Golani, I., Mitra, P. P. (October 2007) Analysis of the Trajectory of Drosophila melanogaster in a Circular Open Field Arena. PLoS ONE, 2 (10). e1083. ISSN 1932-6203 (Electronic)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/17957265
DOI: 10.1371/journal.pone.0001083

Abstract

BACKGROUND: Obtaining a complete phenotypic characterization of a freely moving organism is a difficult task, yet such a description is desired in many neuroethological studies. Many metrics currently used in the literature to describe locomotor and exploratory behavior are typically based on average quantities or subjectively chosen spatial and temporal thresholds. All of these measures are relatively coarse-grained in the time domain. It is advantageous, however, to employ metrics based on the entire trajectory that an organism takes while exploring its environment. METHODOLOGY/PRINCIPAL FINDINGS: To characterize the locomotor behavior of Drosophila melanogaster, we used a video tracking system to record the trajectory of a single fly walking in a circular open field arena. The fly was tracked for two hours. Here, we present techniques with which to analyze the motion of the fly in this paradigm, and we discuss the methods of calculation. The measures we introduce are based on spatial and temporal probability distributions and utilize the entire time-series trajectory of the fly, thus emphasizing the dynamic nature of locomotor behavior. Marginal and joint probability distributions of speed, position, segment duration, path curvature, and reorientation angle are examined and related to the observed behavior. CONCLUSIONS/SIGNIFICANCE: The measures discussed in this paper provide a detailed profile of the behavior of a single fly and highlight the interaction of the fly with the environment. Such measures may serve as useful tools in any behavioral study in which the movement of a fly is an important variable and can be incorporated easily into many setups, facilitating high-throughput phenotypic characterization.

Item Type: Paper
Additional Information: DOI 10.1371/journal.pone.0001083 10.1371/journal.pone.0001083
Subjects: organism description > animal > insect > Drosophila
Investigative techniques and equipment > optical devices
CSHL Authors:
Communities: CSHL labs > Mitra lab
Depositing User: CSHL Librarian
Date: 24 October 2007
Date Deposited: 02 Sep 2011 20:36
Last Modified: 03 Feb 2017 16:40
PMCID: PMC2031922
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15267

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