Czech, B., Malone, C. D., Zhou, R., Stark, A., Schlingeheyde, C., Dus, M., Perrimon, N., Kellis, M., Wohlschlegel, J. A., Sachidanandam, R., Hannon, G. J., Brennecke, J. (2008) An endogenous small interfering RNA pathway in Drosophila. Nature, 453 (7196). pp. 798-802. ISSN 0028-0836
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Abstract
Drosophila endogenous small RNAs are categorized according to their mechanisms of biogenesis and the Argonaute protein to which they bind. MicroRNAs are a class of ubiquitously expressed RNAs of 22 nucleotides in length, which arise from structured precursors through the action of Drosha - Pasha and Dicer- 1-Loquacious complexes(1-7). These join Argonaute-1 to regulate gene expression(8,9). A second endogenous small RNA class, the Piwi-interacting RNAs, bind Piwi proteins and suppress transposons(10,11). Piwi- interacting RNAs are restricted to the gonad, and at least a subset of these arises by Piwi- catalysed cleavage of single-stranded RNAs12,13. Here we show that Drosophila generates a third small RNA class, endogenous small interfering RNAs, in both gonadal and somatic tissues. Production of these RNAs requires Dicer- 2, but a subset depends preferentially on Loquacious(1,4,5) rather than the canonical Dicer- 2 partner, R2D2 ( ref. 14). Endogenous small interfering RNAs arise both from convergent transcription units and from structured genomic loci in a tissue- specific fashion. They predominantly join Argonaute- 2 and have the capacity, as a class, to target both protein- coding genes and mobile elements. These observations expand the repertoire of small RNAs in Drosophila, adding a class that blurs distinctions based on known biogenesis mechanisms and functional roles.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | C-ELEGANS MICRORNA MATURATION ARGONAUTE PROTEINS DISTINCT ROLES BIOGENESIS INITIATION GERMLINE ELEMENTS REVEALS VIRUSES |
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab CSHL labs > Sachidanandam lab School of Biological Sciences > Publications |
| Depositing User: | Tom Adams |
| Date: | 2008 |
| Date Deposited: | 25 Aug 2011 18:13 |
| Last Modified: | 22 Sep 2014 15:58 |
| PMCID: | PMC2895258 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/7713 |
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