Chiu, S. L., Chen, C. M., Cline, H. T. (June 2008) Insulin receptor signaling regulates synapse number, dendritic plasticity, and circuit function in vivo. Neuron, 58 (5). pp. 708-719.
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Abstract
Insulin receptor signaling has been postulated to play a role in synaptic plasticity; however, the function of the insulin receptor in CNS is not clear. To test whether insulin receptor signaling affects visual system function, we recorded light-evoked responses in optic tectal neurons in living Xenopus tadpoles. Tectal neurons transfected with dominant-negative insulin receptor (dnIR), which reduces insulin receptor phosphorylation, or morpholino against insulin receptor, which reduces total insulin receptor protein level, have significantly smaller light-evoked responses than controls. dnIR-expressing neurons have reduced synapse density as assessed by EM, decreased AMPA mEPSC frequency, and altered experience-dependent dendritic arbor structural plasticity, although synaptic vesicle release probability, assessed by paired-pulse responses, synapse maturation, assessed by AMPA/NMDA ratio and ultrastructural criteria, are unaffected by dnIR expression. These data indicate that insulin receptor signaling regulates circuit function and plasticity by controlling synapse density.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DEVBIO MOLNEURO SIGNALING PATHWAY |
| Subjects: | organism description > animal > Frog bioinformatics > genomics and proteomics > analysis and processing > NETBAG ?? Q1 ?? organism description > animal > developmental stage > child bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase |
| CSHL Authors: | |
| Communities: | CSHL labs > Cline lab School of Biological Sciences > Publications |
| Depositing User: | Tom Adams |
| Date: | 12 June 2008 |
| Date Deposited: | 13 Jul 2011 20:43 |
| Last Modified: | 22 Sep 2014 15:54 |
| PMCID: | PMC3057650 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/7709 |
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