Structural dynamics between Argonaute-2 and CK1α promote target RNA release in microRNA-mediated silencing

Garg, Ankur, Braviner, Leah, Axhemi, Armend, Bibel, Brianna, Joshua-Tor, Leemor (March 2026) Structural dynamics between Argonaute-2 and CK1α promote target RNA release in microRNA-mediated silencing. bioRxiv. ISSN 2692-8205 (Submitted)

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Abstract

Argonaute (Ago) proteins associate with 20-22 nucleotide (nt) long microRNAs (miRNAs) to constitute the functional RISC core and downregulate mRNAs containing complementarity to the seed sequence 1-3 . Target RNA engagement in RISC stimulates CK1α-mediated phosphorylation of the conserved eukaryotic insertion (EI) in Ago, releasing the target and enabling the RISC complex to suppress additional target sites for efficient miRNA-mediated silencing 4-6 . Here, we provide a complete structural view of miRNA guide and target binding to human Ago2, showing Ago2 holding the double-stranded guide-target RNA in an untwisted conformation at its center. We visualize the dynamic changes that RISC undergoes as the guide supplementary region progressively base pairs with the target, enabling CK1α binding. Following seed-helix assembly, initial supplementary pairing restricts RISC to a "closed" form, while with half-supplementary pairing, the PAZ domain moves to open RISC to become receptive to CK1α, exhibiting an initial increase in Ago2 phosphorylation. Complete supplementary pairing supports a full PAZ-CK1α interface, allowing for hierarchical phosphorylation of the EI. The combination of target repulsion by EI phosphorylation with an unwound guide-target enables efficient RISC turnover.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > Mapping and Rendering
bioinformatics > genomics and proteomics > Mapping and Rendering > RNA Structure Rendering
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
CSHL Post Doctoral Fellows
School of Biological Sciences > Publications
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 25 March 2026
Date Deposited: 06 Apr 2026 11:59
Last Modified: 06 Apr 2026 11:59
PMCID: PMC13041839
Related URLs:
URI: https://repository.cshl.edu/id/eprint/42143

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