A sub-set of guanine- and cytosine-rich genes are actively transcribed at the nuclear lamin B1 region

Balasooriya, Gayan I, Naik, Payal, Wee, Tse-Luen, Spector, David L (January 2026) A sub-set of guanine- and cytosine-rich genes are actively transcribed at the nuclear lamin B1 region. Cellular and Molecular Life Sciences, 83 (1). p. 51. ISSN 1420-682X (Public Dataset)

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Abstract

Chromatin organization in the mammalian cell nucleus plays a vital role in the regulation of gene expression. The lamina-associated domain at the inner nuclear membrane has been shown to harbor heterochromatin, while the nuclear interior has been shown to contain most of the euchromatin. Here, we show that a sub-set of actively transcribing genes, marked by RNA Pol II pSer2, are associated with Lamin B1 at the inner nuclear envelope in mouse embryonic stem cells (mESCs) and the number of genes proportionally increases upon in vitro differentiation of mESC to olfactory precursor cells. These nuclear periphery-associated actively transcribing genes primarily represent housekeeping genes, and their gene bodies are significantly enriched with guanine and cytosine compared to genes actively transcribed at the nuclear interior. We found the promoters of these gene's to also be significantly enriched with guanine and to be predominantly regulated by zinc finger protein transcription factors. We provide evidence supporting the emerging notion that the Lamin B1 region is not solely transcriptionally silent.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
CSHL Authors:
Communities: CSHL Cancer Center Program > Gene Regulation and Inheritance Program
CSHL labs > Spector lab
CSHL labs > Wee Lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 3 January 2026
Date Deposited: 11 Feb 2026 15:18
Last Modified: 11 Feb 2026 15:18
PMCID: PMC12804584
Related URLs:
Dataset ID:
URI: https://repository.cshl.edu/id/eprint/42069

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