Roche, B, Martienssen, RA (September 2025) RiboCop surveils pre-rRNA processing by Dicer in cellular quiescence. bioRxiv. ISSN 2692-8205 (Public Dataset) (Submitted)
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Abstract
In nature, most cells exist in a quiescent G0 state in which cellular homeostasis must be rigorously maintained in the absence of cell division. Non-coding RNAs are prevalent in G0 and are important regulators of development and differentiation, but their function in quiescence is unclear. Here, we identify pre-rRNA as a direct target of the RNase III enzyme Dicer specifically in quiescence. Dicer is physically present at the rDNA, and improper rRNA processing in mutants results in a nucleolar stress response involving a novel trans-acting non-coding RNA (RiboCop) in complex with the highly conserved proteins Enp2/NOL10 and RNase H1. RiboCop is complementary to unprocessed pre-rRNA and triggers rDNA repeat silencing via Sir2, RENT, and histone H3-lysine-9 (H3K9) methylation. Thus RiboCop silences rDNA specifically during dormancy, when silencing of non-functional rRNA becomes essential.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics |
| CSHL Authors: | |
| Communities: | CSHL labs > Martienssen lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 18 September 2025 |
| Date Deposited: | 06 Oct 2025 12:42 |
| Last Modified: | 06 Oct 2025 12:42 |
| PMCID: | PMC12458480 |
| Related URLs: | |
| Dataset ID: |
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| URI: | https://repository.cshl.edu/id/eprint/41975 |
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