Stillman, Bruce, Diffley, John FX, Iwasa, Janet H (June 2025) Mechanisms for licensing origins of DNA replication in eukaryotic cells. Nature Structural & Molecular Biology. ISSN 1545-9993
Abstract
The initiation of DNA replication in eukaryotic cells begins with the assembly of pre-replicative complexes (pre-RCs) at many sites along each chromosome during the G1 phase of the cell cycle. Pre-RCs license each chromosome for duplication during S phase and mark the origins of DNA replication. In this Review, we discuss and contextualize recent findings identifying the mechanisms of origin recognition and pre-RC assembly mediated by the origin recognition complex (ORC), Cdc6 and the Mcm2-Mcm7 (Mcm2-7) hexamer bound to Cdt1. We also present comprehensive videos that demonstrate the multiple mechanisms for pre-RC assembly and compare the structures of the complexes involved in human and Saccharomyces cerevisiae cells.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| SWORD Depositor: | CSHL Elements |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Elements |
| Date: | 30 June 2025 |
| Date Deposited: | 01 Jul 2025 15:07 |
| Last Modified: | 30 Sep 2025 17:21 |
| PMCID: | PMC12335275 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41897 |
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