Mechanisms for licensing origins of DNA replication in eukaryotic cells

Stillman, Bruce, Diffley, John FX, Iwasa, Janet H (June 2025) Mechanisms for licensing origins of DNA replication in eukaryotic cells. Nature Structural & Molecular Biology. ISSN 1545-9993

Abstract

The initiation of DNA replication in eukaryotic cells begins with the assembly of pre-replicative complexes (pre-RCs) at many sites along each chromosome during the G1 phase of the cell cycle. Pre-RCs license each chromosome for duplication during S phase and mark the origins of DNA replication. In this Review, we discuss and contextualize recent findings identifying the mechanisms of origin recognition and pre-RC assembly mediated by the origin recognition complex (ORC), Cdc6 and the Mcm2-Mcm7 (Mcm2-7) hexamer bound to Cdt1. We also present comprehensive videos that demonstrate the multiple mechanisms for pre-RC assembly and compare the structures of the complexes involved in human and Saccharomyces cerevisiae cells.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
CSHL Authors:
Communities: CSHL labs > Stillman lab
SWORD Depositor: CSHL Elements
Highlight: Stillman, Bruce W.
Depositing User: CSHL Elements
Date: 30 June 2025
Date Deposited: 01 Jul 2025 15:07
Last Modified: 01 Jul 2025 15:07
Related URLs:
URI: https://repository.cshl.edu/id/eprint/41897

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