The regulation of reporter transgene expression for diverse biological imaging applications

Battison, Alexandria S, Merrill, Joseph R, Borniger, Jeremy C, Lyons, Scott K (March 2025) The regulation of reporter transgene expression for diverse biological imaging applications. npj Imaging, 3 (1). ISSN 2948-197X

[thumbnail of 10.1038.s44303-025-00070-6.pdf] PDF
10.1038.s44303-025-00070-6.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.
Download (4MB)

Abstract

A common strategy in biological research is to use the expression of a reporter transgene to non-destructively visualize or measure a biological parameter or process that is otherwise not visible or readily detectable. Genetic reporters have greatly impacted practically all fields of biological research, from visualizing biology in bacteria and microbes, through plant biology, to preclinical research in higher eukaryotes. Fundamentally, many reporter gene assays are reliant upon the expression of a relatively small number of transgenes (e.g., green fluorescent protein (GFP) or firefly luciferase (Fluc)), however, by regulating their expression in different ways, it is possible to generate enormous diversity in the specific aspects of biology measured. For the purposes of this review, rather than focus on the non-invasive imaging modalities or reporter transgenes themselves (recently reviewed1,2), we will instead discuss how reporter transgene expression can be manipulated to generate a huge variety of biological readouts, with specific emphasis in the fields of preclinical oncology and neuroscience, now coming together in the nascent field of cancer neuroscience.

Item Type: Paper
Subjects: diseases & disorders > cancer
diseases & disorders
neurobiology
neurobiology > neuroscience
CSHL Authors:
Communities: CSHL labs > Borniger lab
CSHL labs > Lyons lab
CSHL Cancer Center Program
CSHL Cancer Center Program > Cellular Communication in Cancer Program
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 4 March 2025
Date Deposited: 07 Mar 2025 18:19
Last Modified: 07 Mar 2025 18:19
Related URLs:
URI: https://repository.cshl.edu/id/eprint/41810

Actions (login required)

Administrator's edit/view item Administrator's edit/view item