Generation and Maintenance of Patient-Derived Endometrial Cancer Organoids

Barbi, Mali, Gowthaman, Divya, Katcher, Arielle, Gorman, Megan, Yueh, Brian, Nizam, Aaron, Chung, Charlie, Akyildiz, Erdogan Oguzhan, Frimer, Marina, Goldberg, Gary L, Beyaz, Semir (October 2024) Generation and Maintenance of Patient-Derived Endometrial Cancer Organoids. Bio-protocol, 14 (20). e5093. ISSN 2331-8325

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Abstract

Endometrial cancer (EC) is the leading cause of gynecologic cancer morbidity and mortality in the U.S. Despite advancements in cancer research, EC death rates are increasing, particularly high-grade endometrial cancers. The development of three-dimensional (3D) patient-derived organoid (PDO) models for EC is crucial, as they provide a more accurate representation of the biological and genetic complexity of a patient's tumor compared to traditional 2D cell lines. Here, we describe a protocol for cultivating PDO models from normal endometrium and EC across different EC subtypes. These EC PDO models can be expanded across multiple passages and facilitate the exploration of tumor behavior and drug responses, thereby advancing our understanding of the disease and potentially leading to more effective and individualized novel therapeutic strategies. Key features • Establishment of patient-derived EC and normal endometrium organoids. • Accurate replication of the various histologic and molecular subtypes of EC within the organoids. • Our approach provides a clinically relevant platform for studying EC development, metastasis, progression, and recurrence. • It offers potential for developing targeted therapeutic interventions tailored to specific EC subtypes. Graphical overview Schematic overview of endometrial cancer and normal organoid preparation from patient-derived samples.

Item Type: Paper
Subjects: diseases & disorders > cancer
diseases & disorders
Investigative techniques and equipment
Investigative techniques and equipment > cell culture > cancer organoids
Investigative techniques and equipment > cell culture
diseases & disorders > cancer > cancer types > endometrial cancer
diseases & disorders > cancer > cancer types
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics and Genomics Program
CSHL labs > Beyaz lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 20 October 2024
Date Deposited: 18 Nov 2024 14:35
Last Modified: 18 Nov 2024 14:35
PMCID: PMC11539961
Related URLs:
URI: https://repository.cshl.edu/id/eprint/41735

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