Molecular bases for the constitutive photomorphogenic phenotypes in Arabidopsis

Pham, Vinh Ngoc, Xu, Xiaosa, Huq, Enamul (December 2018) Molecular bases for the constitutive photomorphogenic phenotypes in Arabidopsis. Development, 145 (23). dev169870. ISSN 0950-1991 (Public Dataset)

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Abstract

The transition from skotomorphogenesis to photomorphogenesis is regulated in part by the COP1/SPA complex and phytochrome-interacting factors (PIFs) in Arabidopsis The constitutive photomorphogenic (cop) phenotypes of cop1 and spaQ mutants have been shown to result from a high abundance of positively acting transcription factors. Here, we show that the four major PIF proteins are unstable in cop1 mutants and that overexpression of PIF1, PIF3, PIF4 and PIF5 suppresses cop1 phenotypes in the dark. A comparison of the transcriptome data among cop1, spaQ and pifQ reveals remarkably overlapping gene expression profiles with preferential regulation of PIF direct target genes. Additionally, HFR1 strongly inhibits the in vivo binding and transcriptional activation activity of PIF1 in the dark. Taken together, these data suggest that the cop phenotypes of the cop1 and spaQ mutants are due to a combination of the reduced level of PIFs, increased levels of positively acting transcription factors (e.g. HY5/HFR1) and the HFR1-mediated inhibition of PIF-targeted gene expression in the dark. This article has an associated 'The people behind the papers' interview.

Item Type:	Paper
Subjects:	organism description > plant > Arabidopsis organism description > plant
CSHL Authors:	Xu, Xiaosa
Communities:	CSHL labs > Jackson lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	3 December 2018
Date Deposited:	11 Sep 2024 17:28
Last Modified:	11 Sep 2024 17:28
PMCID:	PMC6288388
Related URLs:	Publisher
Dataset ID:	GEO: GSE112662 GEO: GSE39217 GEO: GSE43286
URI:	https://repository.cshl.edu/id/eprint/41657

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