Evolutionary conservation of receptor compensation for stem cell homeostasis in Solanaceae plants

Seo, Myeong-Gyun, Lim, Yoonseo, Hendelman, Anat, Robitaille, Gina, Beak, Hong Kwan, Hong, Woo-Jong, Park, Soon Ju, Lippman, Zachary B, Park, Young-Joon, Kwon, Choon-Tak (June 2024) Evolutionary conservation of receptor compensation for stem cell homeostasis in Solanaceae plants. Horticulture Research, 11 (6). uhae126. ISSN 2662-6810 (Public Dataset)

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Abstract

Stem cell homeostasis is pivotal for continuous and programmed formation of organs in plants. The precise control of meristem proliferation is mediated by the evolutionarily conserved signaling that encompasses complex interactions among multiple peptide ligands and their receptor-like kinases. Here, we identified compensation mechanisms involving the CLAVATA1 (CLV1) receptor and its paralogs, BARELY ANY MERISTEMs (BAMs), for stem cell proliferation in two Solanaceae species, tomato and groundcherry. Genetic analyses of higher-order mutants deficient in multiple receptor genes, generated via CRISPR-Cas9 genome editing, reveal that tomato SlBAM1 and SlBAM2 compensate for slclv1 mutations. Unlike the compensatory responses between orthologous receptors observed in Arabidopsis, tomato slclv1 mutations do not trigger transcriptional upregulation of four SlBAM genes. The compensation mechanisms within receptors are also conserved in groundcherry, and critical amino acid residues of the receptors associated with the physical interaction with peptide ligands are highly conserved in Solanaceae plants. Our findings demonstrate that the evolutionary conservation of both compensation mechanisms and critical coding sequences between receptor-like kinases provides a strong buffering capacity during stem cell homeostasis in tomato and groundcherry.

Item Type:	Paper
Subjects:	organism description > plant > Arabidopsis Investigative techniques and equipment organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions Investigative techniques and equipment > CRISPR-Cas9 organs, tissues, organelles, cell types and functions organism description > plant organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:	Hendelman, Anat Robitaille, Gina Lippman, Zachary B.
Communities:	CSHL labs > Lippman lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	June 2024
Date Deposited:	08 Jul 2024 12:38
Last Modified:	08 Jul 2024 12:38
PMCID:	PMC11197305
Related URLs:	Publisher
Dataset ID:	PRJNA491365 PRJNA704671 PRJNA862958
URI:	https://repository.cshl.edu/id/eprint/41593

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