Hackett, Jennifer, Pepenella, Sharon, Marco, Cristina Fernandez, Bodt, Louise, Grajales, Lina Ruiz, Petracca, Jeffry, Burke, Justin, Mayle, Allison, Nash, Bruce, Micklos, David A (April 2024) Simple, Robust Invertebrate DNA Barcoding: Chelex-Based DNA Extraction and Optimized COI Amplification. Methods in molecular biology (Clifton, N.J.), 2744. pp. 119-127. ISSN 1064-3745
Abstract
Chelex-based DNA extractions are well suited for student DNA barcoding research because they are simple, safe, and inexpensive and can be performed without specialized laboratory equipment, allowing them to be performed in classrooms or at home. Extracted DNA is stable in Chelex solution for at least a week at ambient temperature, allowing collection of DNA samples from remote students. These extractions provide quality DNA for many taxa and are optimal for barcoding invertebrates, especially in combination with novel cytochrome c oxidase I (COI) primer cocktails and PCR cycling conditions.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment Investigative techniques and equipment > cloning > PCR Investigative techniques and equipment > assays > cloning > PCR Investigative techniques and equipment > assays Investigative techniques and equipment > cloning Investigative techniques and equipment > assays > cloning |
| CSHL Authors: | |
| Communities: | Dolan DNA Learning Center |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | April 2024 |
| Date Deposited: | 20 May 2024 15:37 |
| Last Modified: | 20 May 2024 15:37 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41555 |
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