PpSCARECROW1 (PpSCR1) regulates leaf blade and mid-vein development in Physcomitrium patens

Mohanasundaram, Boominathan, Palit, Shirsa, Bhide, Amey J, Pala, Madhusmita, Rajoria, Kanishka, Girigosavi, Payal, Banerjee, Anjan K (February 2024) PpSCARECROW1 (PpSCR1) regulates leaf blade and mid-vein development in Physcomitrium patens. Plant Molecular Biology, 114 (1). ISSN 0167-4412 (Public Dataset)

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Abstract

In plants, asymmetric cell divisions result in distinct cell fates forming large and small daughter cells, adding to the cellular diversity in an organ. SCARECROW (SCR), a GRAS domain-containing transcription factor controls asymmetric periclinal cell divisions in flowering plants by governing radial patterning of ground tissue in roots and cell proliferation in leaves. Though SCR homologs are present across land plant lineages, the current understanding of their role in cellular patterning and leaf development is mostly limited to flowering plants. Our phylogenetic analysis identified three SCR homologs in moss Physcomitrium patens, amongst which PpSCR1 showed highest expression in gametophores and its promoter activity was prominent at the mid-vein and the flanking leaf blade cells pointing towards its role in leaf development. Notably, out of the three SCR homologs, only the ppscr1 knock-out lines developed slender leaves with four times narrower leaf blade and three times thicker mid-vein. Detailed histology studies revealed that slender leaf phenotype is either due to the loss of anticlinal cell divisions or failure of periclinal division suppression in the leaf blade. RNA-Seq analyses revealed that genes responsible for cell division and differentiation are expressed differentially in the mutant. PpSCR1 overexpression lines exhibited significantly wider leaf lamina, further reconfirming the role in leaf development. Together, our data suggests that PpSCR1 is involved in the leaf blade and mid-vein development of moss and that its role in the regulation of cell division and proliferation is ancient and conserved among flowering plants and mosses.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions
organism description > plant
CSHL Authors:
Communities: CSHL labs > Pedmale lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: February 2024
Date Deposited: 12 Feb 2024 14:18
Last Modified: 12 Feb 2024 14:21
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Dataset ID:
URI: https://repository.cshl.edu/id/eprint/41435

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