Single-Cell Transcription Mapping of Murine and Human Mammary Organoids Responses to Female Hormones

Ortiz, Jenelys Ruiz, Lewis, Steven M, Ciccone, Michael, Chatterjee, Deeptiman, Henry, Samantha, Siepel, Adam, Dos Santos, Camila O (January 2024) Single-Cell Transcription Mapping of Murine and Human Mammary Organoids Responses to Female Hormones. Journal of Mammary Gland Biology and Neoplasia, 29 (1). p. 3. ISSN 1083-3021 (Public Dataset)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/38289401
DOI: 10.1007/s10911-023-09553-x

Abstract

During female adolescence and pregnancy, rising levels of hormones result in a cyclic source of signals that control the development of mammary tissue. While such alterations are well understood from a whole-gland perspective, the alterations that such hormones bring to organoid cultures derived from mammary glands have yet to be fully mapped. This is of special importance given that organoids are considered suitable systems to understand cross species breast development. Here we utilized single-cell transcriptional profiling to delineate responses of murine and human normal breast organoid systems to female hormones across evolutionary distinct species. Collectively, our study represents a molecular atlas of epithelial dynamics in response to estrogen and pregnancy hormones.

Item Type: Paper
Subjects: bioinformatics
diseases & disorders
bioinformatics > genomics and proteomics
Investigative techniques and equipment
organism description > animal
Investigative techniques and equipment > cell culture > cancer organoids
Investigative techniques and equipment > cell culture
bioinformatics > genomics and proteomics > small molecules > estrogen
organism description > animal > mammal
organism description > animal > mammal > rodent > mouse
diseases & disorders > pregnancy
organism description > animal > mammal > rodent
bioinformatics > genomics and proteomics > small molecules
CSHL Authors:
Communities: CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Animal Shared Resource
CSHL Cancer Center Shared Resources > Organoid Service
CSHL Cancer Center Shared Resources > Sequencing Technology & Analysis Service
CSHL Cancer Center Shared Resources > Single-Cell Biology Service
School of Biological Sciences > Publications
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 30 January 2024
Date Deposited: 01 Feb 2024 13:49
Last Modified: 29 Feb 2024 18:52
Related URLs:
Dataset ID:
  • PRJNA1015687
  • https://github.com/dosSantosLabCSHL?tab=repositories
URI: https://repository.cshl.edu/id/eprint/41427

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