Single-Cell Transcription Mapping of Murine and Human Mammary Organoids Responses to Female Hormones

Ortiz, Jenelys Ruiz, Lewis, Steven M, Ciccone, Michael, Chatterjee, Deeptiman, Henry, Samantha, Siepel, Adam, Dos Santos, Camila O (January 2024) Single-Cell Transcription Mapping of Murine and Human Mammary Organoids Responses to Female Hormones. Journal of Mammary Gland Biology and Neoplasia, 29 (1). p. 3. ISSN 1083-3021 (Public Dataset)

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Abstract

During female adolescence and pregnancy, rising levels of hormones result in a cyclic source of signals that control the development of mammary tissue. While such alterations are well understood from a whole-gland perspective, the alterations that such hormones bring to organoid cultures derived from mammary glands have yet to be fully mapped. This is of special importance given that organoids are considered suitable systems to understand cross species breast development. Here we utilized single-cell transcriptional profiling to delineate responses of murine and human normal breast organoid systems to female hormones across evolutionary distinct species. Collectively, our study represents a molecular atlas of epithelial dynamics in response to estrogen and pregnancy hormones.

Item Type:	Paper
Subjects:	bioinformatics diseases & disorders bioinformatics > genomics and proteomics Investigative techniques and equipment organism description > animal Investigative techniques and equipment > cell culture > cancer organoids Investigative techniques and equipment > cell culture bioinformatics > genomics and proteomics > small molecules > estrogen organism description > animal > mammal organism description > animal > mammal > rodent > mouse diseases & disorders > pregnancy organism description > animal > mammal > rodent bioinformatics > genomics and proteomics > small molecules
CSHL Authors:	Ciccone, Michael F Henry, Samantha Siepel, Adam Dos Santos, Camila O. Lewis, Steven M. Chatterjee, Deeptiman Ruiz-Ortiz, Jenelys
Communities:	CSHL Cancer Center Shared Resources > Animal Services CSHL Cancer Center Shared Resources > Animal Shared Resource CSHL Cancer Center Shared Resources > Organoid Service CSHL Cancer Center Shared Resources > Sequencing Technology & Analysis Service CSHL Cancer Center Shared Resources > Single-Cell Biology Service School of Biological Sciences > Publications CSHL Cancer Center Program CSHL Cancer Center Program > Cancer Genetics and Genomics Program
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	30 January 2024
Date Deposited:	01 Feb 2024 13:49
Last Modified:	29 Apr 2024 18:26
Related URLs:	Publisher
Dataset ID:	PRJNA1015687 https://github.com/dosSantosLabCSHL?tab=repositories
URI:	https://repository.cshl.edu/id/eprint/41427

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