Transgenic expression of walleye dermal sarcoma virus rv-cyclin (orfA) in zebrafish does not result in tissue proliferation

Paul, Thomas A, Rovnak, Joel, Quackenbush, Sandra L, Whitlock, Kathleen, Zhan, Huiqing, Gong, Zhiyuan, Spitsbergen, Jan, Bowser, Paul R, Casey, James W (April 2011) Transgenic expression of walleye dermal sarcoma virus rv-cyclin (orfA) in zebrafish does not result in tissue proliferation. Marine Biotechnology, 13 (2). pp. 142-150. ISSN 1436-2228

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Abstract

Walleye dermal sarcoma (WDS) is a benign tumor of walleye fish that develops and completely regresses seasonally. The retrovirus associated with this disease, walleye dermal sarcoma virus, encodes three accessory genes, two of which, rv-cyclin (orfA) and orfb, are thought to play a role in tumor development. In this study, we attempted to recapitulate WDS development by expressing rv-cyclin in chimeric and stable transgenic zebrafish. Six stable transgenic lines expressing rv-cyclin from the constitutive CMVtk promoter were generated. Immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction demonstrate that rv-cyclin is widely expressed in different tissues in these fish. These lines were viable and histologically normal for up to 2 years. No increase in tumors or tissue proliferation was observed following N-ethyl N-nitrosourea exposure or following tail wounding and subsequent tissue regeneration compared to controls. These data indicate that rv-cyclin is not independently sufficient for tumor induction in zebrafish.

Item Type:	Paper
Subjects:	bioinformatics diseases & disorders > cancer bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification diseases & disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics diseases & disorders > neoplasms organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation organs, tissues, organelles, cell types and functions > cell types and functions organism description > animal > fish bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organs, tissues, organelles, cell types and functions organism description > virus > retrovirus diseases & disorders > cancer > cancer types > sarcoma diseases & disorders > cancer > cancer types organism description > virus organism description > animal > fish > zebrafish
CSHL Authors:	Zhan, Huiqing
Communities:	CSHL labs > Zador lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	April 2011
Date Deposited:	22 Jan 2024 20:56
Last Modified:	22 Jan 2024 20:56
PMCID:	PMC3364296
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41411

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