The maize PLASTID TERMINAL OXIDASE (PTOX) locus controls the carotenoid content of kernels

Nie, Yongxin, Wang, Hui, Zhang, Guan, Ding, Haiping, Han, Beibei, Liu, Lei, Shi, Jian, Du, Jiyuan, Li, Xiaohu, Li, Xinzheng, Zhao, Yajie, Zhang, Xiaocong, Liu, Changlin, Weng, Jianfeng, Li, Xinhai, Zhang, Xiansheng, Zhao, Xiangyu, Pan, Guangtang, Jackson, David, Li, Qin-Bao, Stinard, Philip S, Arp, Jennifer, Sachs, Martin M, Moose, Steven, Hunter, Charles T, Wu, Qingyu, Zhang, Zhiming (January 2024) The maize PLASTID TERMINAL OXIDASE (PTOX) locus controls the carotenoid content of kernels. The Plant Journal. ISSN 0960-7412

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Abstract

Carotenoids perform a broad range of important functions in humans; therefore, carotenoid biofortification of maize (Zea mays L.), one of the most highly produced cereal crops worldwide, would have a global impact on human health. PLASTID TERMINAL OXIDASE (PTOX) genes play an important role in carotenoid metabolism; however, the possible function of PTOX in carotenoid biosynthesis in maize has not yet been explored. In this study, we characterized the maize PTOX locus by forward- and reverse-genetic analyses. While most higher plant species possess a single copy of the PTOX gene, maize carries two tandemly duplicated copies. Characterization of mutants revealed that disruption of either copy resulted in a carotenoid-deficient phenotype. We identified mutations in the PTOX genes as being causal of the classic maize mutant, albescent1. Remarkably, overexpression of ZmPTOX1 significantly improved the content of carotenoids, especially β-carotene (provitamin A), which was increased by ~threefold, in maize kernels. Overall, our study shows that maize PTOX locus plays an important role in carotenoid biosynthesis in maize kernels and suggests that fine-tuning the expression of this gene could improve the nutritional value of cereal grains.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > plant > maize organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > plasmid
CSHL Authors:	Jackson, David P.
Communities:	CSHL labs > Jackson lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	10 January 2024
Date Deposited:	16 Jan 2024 14:38
Last Modified:	16 Jan 2024 14:38
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41399

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