A large sequenced mutant library - valuable reverse genetic resource that covers 98% of sorghum genes

Jiao, Yinping, Nigam, Deepti, Barry, Kerrie, Daum, Chris, Yoshinaga, Yuko, Lipzen, Anna, Khan, Adil, Parasa, Sai-Praneeth, Wei, Sharon, Lu, Zhenyuan, Tello-Ruiz, Marcela K, Dhiman, Pallavi, Burow, Gloria, Hayes, Chad, Chen, Junping, Brandizzi, Federica, Mortimer, Jenny, Ware, Doreen, Xin, Zhanguo (December 2023) A large sequenced mutant library - valuable reverse genetic resource that covers 98% of sorghum genes. The Plant Journal. ISSN 0960-7412

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Abstract

Mutant populations are crucial for functional genomics and discovering novel traits for crop breeding. Sorghum, a drought and heat-tolerant C4 species, requires a vast, large-scale, annotated, and sequenced mutant resource to enhance crop improvement through functional genomics research. Here, we report a sorghum large-scale sequenced mutant population with 9.5 million ethyl methane sulfonate (EMS)-induced mutations that covered 98% of sorghum's annotated genes using inbred line BTx623. Remarkably, a total of 610 320 mutations within the promoter and enhancer regions of 18 000 and 11 790 genes, respectively, can be leveraged for novel research of cis-regulatory elements. A comparison of the distribution of mutations in the large-scale mutant library and sorghum association panel (SAP) provides insights into the influence of selection. EMS-induced mutations appeared to be random across different regions of the genome without significant enrichment in different sections of a gene, including the 5' UTR, gene body, and 3'-UTR. In contrast, there were low variation density in the coding and UTR regions in the SAP. Based on the Ka /Ks value, the mutant library (~1) experienced little selection, unlike the SAP (0.40), which has been strongly selected through breeding. All mutation data are publicly searchable through SorbMutDB (https://www.depts.ttu.edu/igcast/sorbmutdb.php) and SorghumBase (https://sorghumbase.org/). This current large-scale sequence-indexed sorghum mutant population is a crucial resource that enriched the sorghum gene pool with novel diversity and a highly valuable tool for the Poaceae family, that will advance plant biology research and crop breeding.

Item Type:	Paper
Subjects:	organism description > plant behavior organism description > plant behavior > crop yield improvement organism description > plant organism description > plant > sorghum
CSHL Authors:	Wei, Xuehong Lu, Zhenyuan Ware, Doreen H. Tello-Ruiz, Marcella
Communities:	CSHL labs > Ware lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	15 December 2023
Date Deposited:	20 Dec 2023 16:39
Last Modified:	20 Dec 2023 16:39
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41324

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